Strategic And Cultural Challenges Enablers â€Myassignmenthelp.Com

Question: Examine About The Strategic And Cultural Challenges Enablers? Answer: Introducation The accompanying report is planned for depicting the authoritative structure of Woolworths, the main food retailer in Australia and the issues that are related with its tasks because of the structure embraced by the association. From that point, the report would be coordinated towards getting an outline of the potential framework procurement techniques, deals flowchart, control issues and conceivable outcomes of misrepresentation that can be seen in framework picked for the association. Data frameworks are seen as a significant corporate resource inferable from the more significant expense of the frameworks and the advantages encouraged by them. The need for basic examination of the accessible choices in the space of data frameworks obtaining could be seen as a supporter of the drawn out help for business activities. The noticeably watched wellsprings of frameworks securing that can serve the vital data needs of the association are seen in outside or in-house arrangements. The flowchart for the business strategy would give a neat impression of one of huge parts of vital data. The comprehension of the business system permits the fear of unmistakable wellsprings of income that can be caught in the particular phases of the business technique. From that point, the association should likewise consider the particular restrictions which could be seen on account of controls and issues of extortion that could be related with the data framework. Hierarchical structure: The hierarchical structure of Woolworths is seen to be a level authoritative structure with negligible layers of chain of importance. The trademark component of centralization could be seen through announcing of the lower levels of the executives to the CEO of the association (Amrollahi, Ghapanchi Najaftorkaman, 2014). The improvement of the effectiveness of authoritative workforce could be expected as a plausible result from the level hierarchical structure which could be noted from the decrease of center layers of the executives. The remarkable bit of leeway that can be drawn from the prohibition of center administration alludes to the entrance to human capital that can be diverted towards other pivotal useful territories associated with the top administration and ground the board (Arvidsson, Holmstrm Lyytinen, 2014). The association gets a significant preferred position from the level hierarchical structure through expanding adaptability of correspondence among the senior administration and store staff. The various ramifications that can be attracted setting of the level hierarchical structure additionally alludes to the associations among clients and workers at the customer facing facade just as the faculty engaged with the deals and advertising division of Woolworths. Woolworths has continued its notoriety in the area of food retail division in Australia close by tending to the varieties in client inclinations and the dynamic changes saw in the outer condition with the assistance of its level authoritative structure (Haron, Sabri Zolkarnain, 2013). The prerequisite of a key data framework is required for Woolworths so as to address the slowly extending activities of Woolworths which could bolster the current framework foundation of the association so as to encourage productivity in useful regio ns, for example, gracefully chain tasks and stock administration. Operational issues: The operational issues that could be foreseen on account of Woolworths because of its authoritative structure include noticeable references to the constraint of control benefits for the associations the executives. Since the tasks of Woolworths are executed for an enormous scope in Australia, the associations the executives requires adaptable assignment of duties to administrators and representatives just as compelling appropriation of hierarchical goals (Henriques de Gusmo Pereira Medeiros, 2016). The distribution of duties could get uncertain since the level hierarchical structure doesn't suggest any opportunities for formal correspondence channels that could prompt significant operational issues. Another significant operational issue that can be seen on account of Woolworths alludes to the administration of HR since the human capital is answerable for exploring the choices that are proposed by the senior administration. This factor prompts significant issues in the viable acknowledgment of control suggestions in the administration of store level staff close by the worries for proficient connections and the individual communication among workers and the administration. The centrality of individual connections could be expected as significant ramifications for creating responsibility of workers for their expert destinations and obligations close by the expansion of trust in the association among representatives (Jin, et al., 2014). Woolworths is likewise at risk to encounter inconvenient outcomes in its activities because of the hierarchical structure, for example, issues emerging out of intensity clashes and deterioration of worker inspiration. The basic survey of these operational issues is fundamental to secure the prerequisites for a key data framework for the Woolworths (Lee, et al., 2015). Frameworks securing strategy: The determination of a reasonable framework obtaining strategy is vital for Woolworths so as to help the association in tending to operational issues distinguished if there should be an occurrence of the association. The two conspicuously known strategies for framework obtaining include outer and inner framework improvement instruments. The outer wellsprings of sending a key data framework could be seen in re-appropriating, distributed computing, open source applications and off-shoring rehearses (Osman, El Beltagi Hardaker, 2015). Bookkeeping to Parry, De Bock White, interior data frameworks improvement could be guaranteed through utilizing an in-house answer for building up the data framework. The interior data frameworks improvement approach could be arranged into the unmistakable strategies forever cycle advancement and fast application improvement (Parry, De Bock White, 2015). The favored framework securing technique that can be applied on account of Woolworths is through outside strategy for redistributing that would empower it to procure an exceptionally created data framework which could help the association in acknowledging cost adequacy nearby providing food the data the executives needs, for example, adaptable recovery of stock data from circulation focuses that would empower representatives to work continuously. Deals strategy: According to Rahman, the business strategy flowchart is considered as a urgent asset for an association to diagram a framework for guaranteeing offer of items encouraged by Woolworths. The accentuation on the business technique could be secured from the factor that deals of a result of administration connote the advancement of connection among client and the specialist co-ops (Rahman, 2016). The business method on account of Woolworths is started with commercial and promoting activities to expand mindfulness in regards to the items. The commercial procedure is trailed by client acknowledgment and assurance of estimating to address distinctive client fragments. It is basic to comprehend the evaluating is trailed by clients examination of the item as indicated by different quality measurements. The last stage in the business method is the buying of item by client. Control issues: The control gives that could be recognized for Woolworths alludes to the ramifications of information vulnerabilities saw in security systems open for the association. The potential outcomes that could be drawn from the authoritative structure of Woolworths in setting of dangers of misrepresentation include the job of relationship among various divisions of the association. According to Salleh, et , the interrelationship between the various divisions of Woolworths is additionally intelligent of the plan changes in the key data framework inferring absence of access controls. In this way, secret data could be effectively lost prompting adverse ramifications for Woolworths which makes the need to get ready emergency courses of action for these dangers for getting tenable results from the data frameworks (Salleh, et al., 2015). The goals of monetary resource the board in contemporary business situation are to a great extent tended to by improvement in PCs just as data and correspondence innovation. The expansion of bookkeeping data frameworks have not just given dependable chances to adaptable documentation and the executives of records in associations yet additionally gave offices to checking of income (Sugiharto, 2016). The bookkeeping data frameworks are considered as valid assets to screen the usage and execution of money related resources of an association subsequently giving chances to long haul food in the commercial center. Different examination studies and auxiliary data relating to bookkeeping data frameworks have shown their viability in giving dependability and command over money related administration of an association. Improvement and appropriation of bookkeeping programming bundles: The bookkeeping programming bundles could be comprehensively portrayed as application programming planned for documentation and the board of the distinctive bookkeeping exchanges identified with the association, for example, creditor liabilities, charging, money due and general records. As indicated by Tarigan Kraus, the product bundle additionally serves the job of a vital data framework through aiding rearrangements of the money related administration procedure of an endeavor. The turn of events and selection of bookkeeping programming bundles are to a great extent dependent on the business in which the orga

YouTube increases company Essay Example for Free

YouTube builds organization Essay Presentation These days, various individuals invest a great deal of energy utilizing the Internet. In this way, numerous organizations contribute generous capital onInternet promoting to expand their income. YouTube is a valuable technique to promote organization produce, since 3 billion individuals watch YouTube consistently (Henry. 2011). There are diverse typesof client, such huge numbers of organizations center around YouTube for this market. Notwithstanding, a few people think YouTube is certifiably not a valuable strategy for promoting; in light of the fact that numerous individuals may overlook the 15 seconds obligatory video ads or may not focus on the video ads (Fisher. 2011). This examination thought about whether YouTube is a decent publicizing technique to urge individuals to purchase new items, to help organization to expands income. Points Discover how YouTube expands organization income See if YouTube changes people’s shopping propensities See whether YouTube is a helpful promoting instrument for an organization 1.2 Objectives Offer guidance to organizations with regards to how YouTube might be utilized to expand organization income Offer exhortation as to manners by which YouTube may impact changes in shopping propensities Strategy for Research The undertaking depends on optional research and essential research. Optional research data was utilized to pick up data about YouTube and Internet promoting from scholastic books, diaries, papers and sites. A study was led so as to find the demeanor and assessments of the overall population. A survey was utilized as, as indicated by McFedries (2008) polls are viewed as the best and proficient method of leading this kind of essential research since it permits essential information and data from the interviewees straightforwardly. The respondents comprised of 20 global understudies going to LSI school in Portsmouth. It was led on the twentieth May 2013. Discoveries from auxiliary research YouTube is one of the best sites on the planet. Numerous individuals may watch and tune in to this site. Accordingly, YouTube can be a viable publicizing medium in advertising. In this manner, numerous organizations spend a great deal ofâ money on YouTube to build their income. It is conceivable that this type of promoting can change people’s shopping propensity as, while they are taking a gander at YouTube data, they are presented to the publicizing cuts, which go with the data. Nonetheless, the facts may confirm that individuals have the decision to overlook the promoting and simply center around the data they need to watch. For this situation, there is banter about whether the publicizing is squandered. All things considered, there appears to adequate proof to exhibit that the cash spent on such promoting is beneficial. Right off the bat, YouTube net income was $2.6 billion out of 2012 an expansion of 35% contrasted with the second quarter of 2011 (Larry. 2012). From this outcome it very well may be seen that numerous organizations spend a great deal of cash on YouTube promoting, in light of the fact that in this age, time spent on PCs is expanding and numerous individuals discover some amusement from YouTube. 3 billion individuals watch YouTube consistently (Henry. 2011). Numerous organizations can see that YouTube has various kinds of client. So it is a possibly helpful instrument to advance their organization. For instance, VEVO is the main organization that has real music channels on YouTube and it has numerous endorsers. Thus, when individuals watch a music video, it will incorporate publicizing about that music or different items. At the point when individuals are keen on that music or items they can get more data and get them. Also, YouTube will make an examination of each client, for example, what they regularly see. At that point YouTube will deal with the information and will propose some comparable video to clients. Additionally, YouTube will give the information to certain organizations which are identified with that video to do some exploration educate them about client propensities. At that point, a few organizations will include publicizing in that video let clients realize their organization has a few items that are reasonable for them and change customer’s shopping propensities (YouTube. 2013). At last, numerous individuals don't trust YouTube publicizing achievability, in light of the fact that numerous individuals may simply watch the 15 seconds at that point disregard it. Along these lines, numerous individuals question YouTube publicizing. With the expanding accessibility of advances in innovation, the promoting procedures have improved and may urge a bigger number of guests to watch the adverts as opposed to overlook them. As indicated by an ongoing exploration did by Fisher (2011), just 30% of YouTube ads are skipped. Discoveries from Primary Research This exploration utilized an example of 20 individuals, those are LSI understudies andâ teacher,20 polls were returned and the interviewees responded to all inquiries. As indicated by the outcomes from the main inquiry which posed to individuals which site they for the most part use to discover new music recordings, it tends to be seen from reference section (b) that 80%chose YouTube to discover new music recordings, likewise 15% utilized Google and just 5% utilized QQ Music, yet no one utilized Yahoo and Facebook to discover the new music video. The subsequent inquiry looked into how much time do individuals spend on normal on YouTube regular, it is appeared by index (c), that close to 35% of interviewees go through 3 to 4 hours on YouTube everyday.Likewise, there were 25% of interviewees who go through 1 to 2 hours out of each day and the quantity of interviewees who go through under 1 hour and more than 4 hours was the equivalent, and it was 20%. The third inquiry posed to individuals about their propensities from before the progressions in 2012, how frequently did they watch a full ad on YouTube, it is appeared in informative supplement (d), that 60% by and large, they justsaw it between 2 to multiple times, the quantity of individuals who saw it between 5 to multiple times and once every multiple times was the equivalent, it was 20%. The fourth inquiry examined the progressions that occurred after 2012, and how oftenpeople observed full adverting on YouTube, it is appeared in reference section (e), that 35% of interviewees saw the promotion that showed up between 5 to multiple times. 30% of interviewees saw it between 8 to multiple times, the quantity of respondents who just observed it between 2 to multiple times and individuals who watched it each time is the equivalent †15%. The fifth inquiry, member concurred that obligatory video commercials are valuable for them, is appeared in supplement (f), the quantity of mem bers who believe are now and then helpful and once in a while exceptionally valuable is the equivalent †35%.Likewise, there were 25 % of member believe are typically extremely valuable and it simply had 5% of member believe are helpful. The 6th inquiry, individuals concurred that obligatory video promotions can build your advantage and therefore purchase publicized items, it very well may be seen from informative supplement (G), 40% of the interviewees concur YouTube compulsory increment their advantage and urge them to purchase the promoted items, though just 5% firmly differ this point. In any case, no one firmly concur that required video ads can expand them intrigue and therefore purchase promoted items. The seventh inquiry, individuals concurred that YouTube could change their shopping propensities, it very well may be seen from reference section (H), the concur rating score of 4 is 35% and the differ rating score of 2 is 20%, speaking to that moreâ interviewees YouTube can change their shopping propensities. Since the level of individuals emphatically concur and unequivocally differ are the equivalent of 5%, an end can't be drawn. The eighth inquiry, have they at any point purchased an item promoted on YouTube in the wake of viewing the video, is appeared in addendum (I), that 60% of interviewees have purchased an item publicized on YouTube subsequent to viewing the video and the staying 40% said they didn't buy the item. The last inquiry is the thing that variables will make them intrigued by the online video notices, is appeared in reference section (J), that 40% of members said they are as of now keen on the item, likewise 20% of participantssaid in light of the fact that the pop stars advertisethe item. Thenumbers of individuals what think's identity is audio effects and they need the items is the equivalent †15%, there are only 10% of participantsare intrigued by the online video notices because of blessing vouchers. Conversation of Findings The review was intended to discover how YouTube increment organization income, regardless of whether YouTube change people’s shopping propensities and see whether YouTube is a helpful promoting instrument for an organization. Initially, the discoveries exhibit that 3 billion individuals watch YouTube consistently (Henry. 2011), the VEVO music organization channel has the most endorsers and has various kinds of clients. So it is a possibly helpful apparatus to advance their organization. From the survey, it very well may be seen from supplement (B)that unmistakably there are 80% interviewees for the most part use YouTube to get some answers concerning new music. Along these lines, YouTube is a succeedmethod to publicize organization produce. Besides, informative supplement (D) and index (E)investigate whether YouTube promoting technique upgrades increment the quantity of watchers. Index (D) speaks to pre promoting system upgrades, when interviewees just generally (60%) see by and large, between 2 to multiple times the commercial showed up. (By and large, between 2 to multiple times the notice showed up decrease to 15%. In like manner, a similar gathering of interviewees will see by and large, between 5 to 7 and 8 to multiple times the promotion showed up increment to 35% and 30%. Likewise it can seen from addendum (G) thatonly 5 % of interviewees unequivocally differ that compulsory video notices can expand intrigue and subsequently purchase promoted items. This aftereffect of this poll is like theâ secondary examine, which underpins that promoting methodologies improved in 2012 urged more individuals to watch the full adverting on YouTube and furthermore expanded people’s enthusiasm to purchase publicized items. Thirdly, it tends to be s

Payday Loan Rollover How Short-Term Loans Turn into Long-Term Debt

Payday Loan Rollover How Short-Term Loans Turn into Long-Term Debt Payday Loan Rollover: How Short-Term Loans Turn Into Long-Term Debt Payday Loan Rollover: How Short-Term Loans Turn Into Long-Term Debt4 out of 5 payday loan borrowers wind up extending their loans and paying much, much more than they expected to than when they originally borrowed.1 How does this happen? Through a harmless-sounding technique called rollover.Why Do People “Need” Payday Loans in the First Place?People who turn to payday loans often have “bad” credit scoreâ€"a FICO score of 630 or less.Not sure what a credit score is? Let Katie Ross, Education and Development Manager for American Consumer Credit Counseling explain. “A credit score is all of the data contained in a credit report, which includes credit history and current account statuses, all compiled into one number using the same method for every consumer so it is standardized. The score is a tool for creditors to quickly assess borrowers to make initial product and interest rate offerings without performing a full credit inquiry.”If you have a low credit score, then you’ ve likely had an experience of getting shut out of from borrowing from traditional institutions like banks or credit unions. And it means that the interest rates you pay on the loans you can get are going to be much higher.Lenders that serve  these “subprime” borrowersâ€"people whose credit scores are below averageâ€"charge those higher rates in order to protect themselves from risk. Since subprime borrowers default on their loans at a higher rate than borrowers with prime scores, lenders risk losing too much money if they charged them normal interest rates.Enter: payday loans.On paper, these are short-term loans with high-interest ratesâ€"the perfect way for someone with poor credit to get cash in a hurry. But in the real word, many payday loans end up trapping borrowers in a cycle of extremely high-cost debt, one from which it can take them years to escape. And the reason for that can be summed up in one simple word: rollover.How does loan rollover work?When a person cannot af ford to pay their payday loan off by the given date, many lenders will give them the opportunity of “rolling over” their loan. It’s basically giving them an extension on the loan’s due date in return for an additional fee.The most common form of loan rollover involves the borrower paying off only the interest owed on their loan. So for a 14-day, $300 loan with a 15 percent interest rate, rolling the loan over would mean paying the lender the $45 owed in interest in order to secure a 14-day extension on the due date.But what about that additional fee? Well, what happens is that the lender then charges the borrower an additional 15 percent in interest on this new, extended term. In one fell swoop, the cost of borrowing for this payday loan jumps from 15 percent to 30 percent. That’s a big jump!And if the borrower is still unable to pay their loan off after the new 14-day term, the lender might have them roll their loan over again.   That’s an additional $45 paid, and an ad ditional 15 percent interest fee charged. The cost of borrowing has now risen to 45 percent, and the borrower is nowhere closer to paying off the original $300 they borrowed.That’s how a “short-term” payday loan can so easily turn into a long-term problem.The cost of rolloverIf the main appeal of payday loans is that they can get you out of debt fast, then rolling a loan over and over again would seem entirely unappealing. And yet, it’s a fact that many payday loan customers end up with long-term debt.According to a 2014 study from the Consumer Financial Protection Bureau (CFPB), 80 percent of all payday loans are the result of rollover or reborrowingâ€"which is the practice of taking out a new payday loan soon after the old one is paid back. (Like rollover, reborrowing is a sign that a given borrower cannot afford to pay back their payday loan.) This begs the question: would the payday loan industry survive if its customers could afford to pay back their loans the first tim e?Maybe not. Another study from the CFPB cites an alarming statistic: the average payday loan customer takes out 10 loans per year and spends almost 200 days in debt. Even though payday loans are sold as a “short-term” debt solution, these numbers point to a pattern of long-term indebtedness.The reason that rollover (and reborrowing) are so common for payday loans likely has something to do with how these loans are structured. Specifically, how they are designed to be paid back all at once. According to a study from the Pew Charitable Trusts, the average payday loan borrower states they can afford about $100 a month towards their loan, even though they owe closer to $430.2 With only a few short weeks to pay back the loan, many payday loan borrowers find that they simply cannot afford to pay the loan back all at once.How to Avoid Payday LoansFolks with bad credit who are considering a payday loan should instead do two things.The first thing they should do is consider out taking a long-term installment loan insteadâ€"as these loans designed to be paid off in a series of small, manageable payments (read more in What’s the Difference Between a Payday Loan and an Installment Loan?). Instead of accruing an additional interest fee every two weeks without ever touching the loan’s principal amount, borrowers who take out an installment loan would be paying both off principal and interest with every payment they make.The second thing these people should do is focus on improving their credit scores. The better their credit, the less likely they are to resort to a payday loan.“Its no secret that consumers with excellent credit have access to their best credit cards and lowest interest rates,” says Chris Piper, Director of Market Strategy for DriveTime Automotive Group. “Having subprime credit can negatively affect your ability to finance a vehicle, own a home and even got a job especially if you will have access to money or its in the finance industry. Slow ly working on improving your credit is imperative to reducing financial stressors in your life.Piper stresses that, “Outside of regularly reviewing their credit reports and scores (and knowing that you should never pay to review your credit reports or scores), subprime consumers should understand what element of their credit history is keeping their score low.”He says that “it might be as simple as an incorrectly reported delinquency, or maybe the utilization on a single credit card is too high and negatively impacting their score. Knowing what the exact reasons why their score is low and practicing good credit hygiene and resolving those issues, when possible, is a sure-fire way to move out of the subprime credit range.”According to Sacha Ferrandi, founder of Source Capital Funding, Inc., “One of the best ways to avoid payday and predatory lenders is to treat credit cards like debit cards, paying back the credit card loan as soon (or shortly after) you make the purchase. This is a great example of borrowing responsibly, as the credit card simply becomes a way to earn points in addition to making a purchase.If you have credit card debt, make sure to pay off that debt on time” says Ferrandi. “Missed payments will severely hurt your credit score and if your credit score drops low enough, financing from a bank will become next to impossible, leaving only payday advances to rely on if an emergency arises.”And even if you still need to take out a loan, choosing a personal installment loan could help you pay your bills and improve your credit at the same time. Certain lenders, like OppLoans, report your payment information to the credit bureaus. Payment history a huge factor in how credit scores are calculated.While there are no “magic bullets” when it comes to raising your score, making your payments on time is a great place to start. Plus, with an installment loan, you’re more likely to have payments you can actually afford. Unlike payday loa ns, installments loans are designed to be paid off the first timeâ€"no rollover required.Works Cited1 CFPB Finds Four Out Of Five Payday Loans Are Rolled Over Or Renewed. ConsumerFinance.gov. Accessed on February 10, 2017 from https://www.consumerfinance.gov/about-us/newsroom/cfpb-finds-four-out-of-five-payday-loans-are-rolled-over-or-renewed/.2 How Borrowers Choose and Repay Payday Loans. PewTrusts.org. Accessed on February 10, 2017 from http://www.pewtrusts.org/~/media/assets/2013/02/20/pew_choosing_borrowing_payday_feb2013-%281%29.pdfAbout the Contributors:Sacha  Ferrandi, is the Founder of Source Capital Funding, Inc. (HardMoneyFirst.com) and is an expert in finance, entrepreneurship, and real estate. Source Capital Funding, Inc., is based in San Diego and operates across the United States.Chris Piper, Director of Market Strategy for DriveTime Automotive Group (DriveTime.com), the nations largest used car dealership network helping people with less-than-perfect credit find and f inance a vehicle.Katie Ross, joined the American Consumer Credit Counseling (ACCC) management team in 2002 and is currently responsible for organizing and implementing high performance development initiatives designed to increase consumer financial awareness. Ms. Rosss main focus is to conceptualize the creative strategic programming for ACCCs client base and national base to ensure a maximum level of educational programs that support and cultivate ACCCs organization.

The World War II And The Cold War - 1247 Words

The term â€Å"military complex† was made popular by Eisenhower who was the president from 1953 to 1961. The term refers to the marriage of military institutions with economic institutions for the reason of securing the nation However, the structure of the intertwined military, government and civilian was started earlier in the century from about 1915. There were many trial and errors to get the ball rolling on such an impacting and controversial issue. Many boundaries had to be overcome for there to function among these industries with the common ground of providing for the nation while at war. Although war’s affect may touch upon many aspects of life not only in America but any nation at war, blurred lines take the place of division between†¦show more content†¦So it is common for the height of the industrial military complex to be focused on the Cold War and World War II. Yet, the beginning years is when important changes took place and forever changed the i nfrastructure of government regarding military and industry. Due to the massive spending dedicated to weapons and warfare, biological and traditional there is even more of vague divergence between the organizations. How this blending came to be regarding commerce and military started around 1917. The first time the United States had to use its economy to prepare for war was for World War I. Production was not only needed for the war but also, to put the economy at full capacity. Therefore the civilian industry had to be enlisted in the production of supplies for the military. A network was created between federal government, military services and industry. Commerce coupled with this new network laid the groundwork for the complex Eisenhower later described in 1961. At these beginning stages, however, there was a disarray of order to accomplish supplying the military with what it demanded. The lack of power to command organization needed to adhere industry with government was owed to the different schools of thought concerning the governmentâ⠂¬â„¢s role in commerce. On one hand it was thought that the government should stay out of the

The Issue Of Police Brutality - 1823 Words

Carson O’Brien 6th Minotaur: An Essay On The Issue Of Police Brutality The poor man lurches towards the nest, a blood trail left by the cut in his foot wanders behind the fellow. His stomach stirs up in a fit, he hasnt eaten in a few days. Footsteps echo throughout the corridor, drowsey the man confuses his own footsteps for those of the creature that defends this cavern. A cold air grazes his neck as he waits to see if the his ears have mistaken something else for the creatures footstep, all is silent only his breath pierces the air. A pidder padder rears it head, the sound of clomping crashes into the man s eardrums as he begins to shake in fear. Chilled to his core he anticipates the worst, but yet in a surprising relief the†¦show more content†¦He would explain to his child that he wouldn’t have food again, as he watches his wife ghost cry to him in his dreams. For what is this is this a dream, is the minotaur real. I don’t know, but one thing that I know is real is that when that boy walked up to the nest those 16 bullets fi red into his chest, head, and stomach were very real.Babe looks upon his handy work, he did his job it’s time for him to set up a crime scene. The blood on his badge remains on the ground next to the boy, yet the blood never washes off either. The floor remains stained, the badge remains stained. The store clerk is frantic, raving about like a madman who just witnessed a murder. Murder, a flimsy word, a inconsistent word, a weak word. Babe returns to his squad car, he calls for his fellow officer for back up. The fellow officers at a rambunctious mix of monsters and men, but if you put them all in a line tell me which one would get more attention from you. Not all police officers are brutal minotaurs, but we unfortunately live in a time where corruption amongst law . men runs rampant, so those who do actually want to serve and protect are forgotten in the shuffle of bullets, hatred, race debates, political outrage, and the death of undeserving americans. Police brutality has been a plague on this nation s people, as well as the reputation of this nation s law enforcement for years. It’s one the biggest

Essay on Abraham Lincoln the Politician - 1473 Words

Abraham Lincoln the Politician Abraham Lincoln: Moral, Just, and Practical Today in the United States one of the most controversial issues is race relations. Mainstream America would like to exist in an environment of integration and race independent societal construct. However, one cannot negate the existence of racially motivated problems. Slavery, which is a primary component of the development of what we call modern American culture and its abolition could be considered the moment where race relations started to become an issue in the US. Slavery was abolished after the civil war by President Abraham Lincoln. The question becomes, was the blood shed in the civil war motivated by a moral imperative to end†¦show more content†¦He built his own flatboat, navigated it to New Orleans, and then settled for a time to manage a store and a mill, and act as Post Master, as well as studying Law. In 1836 he was licensed to practice as an attorney, and settled in the following year in Springfield, Illinois, where he married Mary Todd. Though clashes of temperament between the high tempered Mary and her sincere but often inconsiderate husband meant that the marriage was never an easy one, it was not the tragedy that legend has made it. In 1847 Lincoln was elected to congress as a Whig, but he did not particularly distinguish himself in his one term of office, which was chiefly valuable for the formation of political associations and the establishment of a reputation for sincerity and honesty. He was not reelected, and it was an ebb-period of his career. It was not until 1854 that he began to stand out from the hundreds of provincial lawyer-turned-politician. In the last year the Bilk to reopen the Louisiana purchase to slavery gave rise to much northern opposition, and to the birth of the new Republican party. 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Lincoln has been called many things because of his views fromRead MoreSlavery During The 19th Century895 Words   |  4 PagesStates was split into two sections, the north and south, over the dispute of slavery. The south sought to further slavery while the north was in favor of abolishing slavery. Around 1858, Abraham Lincoln and Stephen Dougla s, two politicians with completely different views on slavery fought each other with politicians trying to win the presidency election of 1860. This was known as the â€Å"Great Debates of 1858†. Slavery during the late 1800’s was at its highest peak; almost 1 out of 4 citizens had slavesRead MoreMaking An Abraham Lincoln Movie901 Words   |  4 Pages Making an Abraham Lincoln movie can be very tricky and a difficult job. Everybody knows the story of Abraham Lincoln and knows the persona that President Lincoln can give out. Spielberg’s, Ford’s, and Griffith’s Lincoln all did very notable work. Griffith’s Lincoln in Birth of a Nation was a worthy performance by Henabery. Ford’s Young Mr. Lincoln was known as one great over all film. The scenes in the film were spot on and the Lincoln played by young Mr. Henry Fonda was the best in the businessRead MoreAbraham Lincoln And The Gettysburg Address1152 Words   |  5 PagesAbraham Lincoln was born on Feb. 12, 1809, in Hardin, now known as larue, County Kentucky. Lincoln got married in 1842 to Mary Todd. His children were Robert Todd, Edward Baker, William Wallace, and Thomas III. A braham was in office from 1834-1842. Abraham Lincoln died at age 56, april 15, 1865. The Republican Party met in Chicago to select a candidate. They took the chance now because of the democrats in turmoil. They needed someone who would help the north and also win most of the electoral collegesRead More Abraham Lincoln Essay1464 Words   |  6 Pages Abraham Lincoln sprang to the presidency from extremely humble and tragic roots. His father, Thomas, and his mother, Nancy, were both illiterate. When Abraham was young, he had a sister who died as an infant and a brother who died as a boy. When Abraham was nine years old, his mother tragically died, leaving him in the care of only his father, who, within the year of his wife’s death, remarried a widow, Sarah Bush Johnston. Sarah was extremely kind to Lincoln and encouraged his developing love ofRead MoreAbraham Lincoln And The Civil War1095 Words   |  5 Pages1861 Abraham Lincoln became the sixteen president of the United States. He had the Proclamation of declaring forever to free slaves within the Confederac y in the year 1863. Lincoln directly told the South In your hands, my dissatisfied fellow countrymen, and not in mine, is the momentous issue of civil war. The government will not assail you.... You have no oath registered in Heaven to destroy the government, while I shall have the most solemn one to preserve, protect and defend it. Lincoln wasRead MoreLincoln and Good Politics Essay946 Words   |  4 PagesLincoln and Good Politics Politicians today as well as yesterday must appeal to the masses to have any chance at being elected into office. Leading up to and during the election of 1860, with tensions rising between the North and South, the issue of slavery was a key to winning the election. Abraham Lincoln had repeatedly affirmed that Congress had no constitutional right to interfere with slavery in the South (Enduring Vision, p. 399). This modest view is what won him the RepublicanRead MoreEssay Abraham Lincoln1426 Words   |  6 Pages As the 16th president, Abraham Lincoln was known to be the greatest American President. He guided his country through one of the most catastrophic experiences in its history, the Civil War. He was a very unique president, being the first president to have a beard, being the tallest president standing at 6’4 and also being the first to be assassinated. Abe Lincoln accomplished many things in the 4 year term he had served before being assassinated. With his intelligence and hard work, he madeRead MoreThe Life Of Abraham Lincoln754 Words   |  4 PagesValenzuela 1 Issac Valenzuela Mrs. Barr 7th Grade Literature May 24, 2016 The Life of Abraham Lincoln Abraham Lincoln was born and raised in a log cabin. He was born on February 12, 1809. His mother was a great Christian. She often told them stories from the Bible. One day, Lincoln’s mother died of poisoned milk when he was only nine. Abraham Lincoln was one of the tallest presidents in our history. He was six feet, four inches tall. When he was elected president, he started theRead MoreAbraham Lincoln, Slavery and the American Civil War Essay1716 Words   |  7 Pagesinvestigation will analyze how Abraham Lincolns view on slavery reflected during and after the American Civil War from 1861 to 1865. To analyze exactly how Lincolns position on slavery affected the war overall, this investigation looks at Lincolns moral and religious views as well as his social and political views. Two main sources were used, both dealing with events relevant to his political career and his roots in his career and other important issues including slavery. Lincoln by David Herbert Do nald

David Beckham free essay sample

This is an essay will be about David Beckham the English soccer player. David is one of the best players in the world. There are several aspects about Beckham`s life. His soccer career, he became more than a usual football player and his personal life. That was the introduction. Now let’s move to the main part. David played in many clubs around the world. However, his most crucial stage of development and reputation happened when he was playing for two of the best Five soccer clubs in the world which are Manchester United from 1993 to 2003 and Real Madrid from 2003 to 2007. Therefore, he won many of championships and individual awards. For instance, he won the Union of European Football Associations (UEFA) Champions league with Manchester United, La Liga with Real Madrid and England player of the year in 2003. Also, he played as a loan player in Ac Milan that is one of the top clubs around the world too. We will write a custom essay sample on David Beckham or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page In addition, in 2007 Beckham had the highest transfer price in his career life when he transferred from Real Madrid to Los Angeles Galaxy by $ 32. 5 million in five years. Beckham became more than an average player. He did not stop just as a famous player, he was smart to use his fame well using. For example, he opened a football academy in London and Los Angeles. Likewise, he became one of the most wanted famous people in the advertising world. He did a large number of advertising for a lot of famous companies such as Pepsi and adidas. Furthermore, Beckham became a model in many famous clothing companies around the world. In 1997, Beckham start dating with the famous singer Victoria, who was one of the spice girls music group. The result for this successful relationship they get married in 1999, at Luttrellstown Castle in Ireland. Now David and Victoria have four children(three sons and a daughter). In conclusion, this essay was about David Beckham the English footballer. It has showed, his football career, Beckham did not stop only as a normal soccer player and his personal life.

Friends Can Bring You Down free essay sample

â€Å"I believe that friends can bring you down, just as much as you hoped they wouldn’t.† I’m almost a 15 year old teenage girl just wanting to be able to make old things better and throw all the feelings away like they don’t matter. It’s been a while now since me and my three other BFF’S (best friends forever) have talked, walking by myself around in the halls and seeing them still together, laughing and not caring that I’m falling apart, tears me down even more than just a little argument that was over in less than five minutes. While sitting at lunch or just floating around all the rumors going on and having the automatic thought â€Å"It’s them†. I don’t understand why if they told me they would always be there when I’m falling apart or need a shoulder to cry on, when they haven’t ever before. We will write a custom essay sample on Friends Can Bring You Down or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page â€Å"Wouldn’t that make you wonder?† knowing the fact that I’m alone and having to move on is also hard to keep my focus. I remember staying the night having slumber party’s was the only thing we all wanted to do every weekend, all niters laughing as hard as we could at nothing. But all I have now is just memories, memories that I think they all forgot about, just like they forgot about me or that I’m still even here. All I have left to do is move on, and not feel sorry anymore there are two other girls Emily (Bonita) and Brittany (Bonita Blonde) that I still have and can have fun with!!! Instead of letting friends bring me down. There’s still a future I believe that I have and I don’t need to sit in my room all the time just to let them feel they have all the power over me. So moving on and finding a way to get over the fact of them not around is gonna take some guts, but I can do it. I think the first thing I’ll try to do is apologize for whatever I did to get it off my chest and let them know how I’m feeling. Cause if you’re like me and you thing you’ve got a good friendship think again or look out because â€Å"friends can bring you down just as much as you hoped they wouldn’t and that’s what I truly believe.†

Scapegoat, Scapegoating, and Scapegoat Theory

Scapegoat, Scapegoating, and Scapegoat Theory Scapegoating refers to a process by which a person or group is unfairly blamed for something that they didnt do and, as a result, the real source of the problem is either never seen or purposefully ignored. Sociologists have documented that scapegoating often occurs between groups when a society is plagued by long-term economic problems or when resources are scarce. Scapegoat theory is used in sociology and psychology as one way to intercept conflict and prejudice between individuals and groups. Origins of the Term The term scapegoat has Biblical origins, coming from the Book of Leviticus. In the book, a goat was sent into the desert carrying the sins of the community. So, a scapegoat was originally understood as a person or animal that symbolically absorbed the sins of others and carried them away from those who committed them. Scapegoats and Scapegoating in Sociology Sociologists recognize four different ways in which scapegoating takes place and scapegoats are created. Scapegoating can be a one-on-one phenomenon, in which one person blames another for something he/she or someone else did. This form of scapegoating is common among children, who blame a sibling or a friend for something they did, to avoid the shame of disappointing their parents and the punishment that might follow a misdeed.Scapegoating also occurs in a one-on-group manner, when one person blames a group for a problem they did not cause: wars, deaths, financial losses of one kind or another, and other personal struggles. This form of scapegoating may sometimes be unfairly blamed on racial, ethnic, religious, class, or anti-immigrant biases.Sometimes scapegoating takes a group-on-one form, when a group of people singles out and blames one person for a problem. For example, when the members of a sports team blame a player who made a mistake for the loss of a match, though other aspects of play also affected the outcome. Or, when someone who alleges an assault is then scapegoated by me mbers of the community for causing trouble or ruining the life of the attacker. Finally, and of most interest to sociologists, is the form of scapegoating that is group-on-group. This occurs when one group blames another for problems that the groups collectively experience, which might be economic or political in nature- like blaming a particular party for the Great Depression (1929-1939) or the Great Recession (2007-2009). This form of scapegoating often manifests across lines of race, ethnicity, religion, or national origin. The Scapegoat Theory of Intergroup Conflict Scapegoating of one group by another has been used throughout history, and still today, as a way to incorrectly explain why certain social, economic, or political problems exist and harm the group doing the scapegoating. Some sociologists say that their research shows that groups that scapegoat occupy a low socio-economic status in society and have little access to wealth and power. They say these people are often experiencing prolonged economic insecurity or poverty, and come to adopt shared outlooks and beliefs that have been documented to lead to prejudice and violence. Sociologists who embrace socialism as a political and economic theory argue that those in a low socioeconomic status are naturally inclined to scapegoat due to the unequal distribution of resources within the society. These sociologists place blame on capitalism as an economic model and exploitation of workers by a wealthy minority. However, these are not the viewpoints of all sociologists. As with any science involving theories, study, research, and conclusions- its not an exact science, and therefore there will be a variety of viewpoints.

Social Phenomena Essay Example | Topics and Well Written Essays - 3000 words

Social Phenomena - Essay Example Each of these views relies upon more or less legitimate arguments and evidence, but neither of them is flawless. Functionalist perspective still remains arguably the most influential tool for understanding the phenomenon of deviant behavior. Since the term 'deviant behavior' is rather broad, only two types of social deviance will be closely analyzed within this project: crime and suicide. Functionalist explanation of the causes and social outcomes of these types can lead us to understanding of functionalist perspective on deviant behavior in general. Emile Durkheim, the famous French sociologist of the 19th - 20th century, was probably the first scholar to adopt the logic of the functional approach studying the phenomenon of deviant behavior. Thus, Durkheim claimed, "When . . . the explanation of a social phenomenon is undertaken, we must seek separately the efficient cause which produces it and the function it fulfills. We must determine whether there is a correspondence between the fact under consideration and the general needs of the social organism, and in what this correspondence consists" (Durkheim, 1930 in Coser, 1977: 143). Ut Utilizing these principles Durkheim focused on the way a society stimulates individuals demonstrate deviant behavior than on individual characteristics of those whom demonstrate such behavior, though majority of sociologists of those days viewed crime, suicide, and other forms of social deviance as an outcome of inborn psychological characteristics of the individual. Durkheim considered such approach flawed. Instead, the scholar argued that deviance in general and crime in particular was by no means abnormal or dangerous for the society: "Crime is normal, an inevitable and necessary part of every society [although] it may take abnormal forms, such as when the crime rate is unusually high (Durkheim, 1961: 872). Durkheim's views on another form of deviance - suicide - were absolutely the same. "La Suicide" was the third major work of Emile Durkheim. This book was the first sociological research that established the traceable empiric connection between the phenomenon of individual suicide and social environment, and defined certain social functions of suicide. In the end of the 19th century suicide or self-destruction was considered a form of deviant behaviour that occurred in insane individuals. Durkheim challenged this traditional standpoint that suicide was an unpreventable pathology. Instead, he provided " a sociological explanation for a phenomenon traditionally regarded as exclusively psychological and individualistic" (Thompson, 1982: 109). In fact, Durkheim formulated the founding principles of functionalist perspective on deviance precisely in his study of suicide. Even today, despite abundance of recent studies, Durkheim's work remains the most significant sociological analysis of s uicide in modern societies. Suicide is a complex phenomenon that can be caused by numerous factors. Many reasons both individual and social have been demonstrated to have certain correlation with self-destructive

Curriculum as process Assignment Example | Topics and Well Written Essays - 250 words

Curriculum as process - Assignment Example The main goal of this transformation was to help students understand democracy and all that it encompasses (Freedman, 2003). Furthermore, it would enable them to appreciate the unity in cultural diversity. Several methods can be used to develop and sustain a democratic curriculum. These include; teaching about conflicts and discussion of issues in open forums by students whereby interests of all individuals should be considered. It is also good to conceptualize curriculum as one process that is concerned with learning in classrooms and outside classroom. Sometimes, schools concentrate on learning in classroom ignoring co-curricular activities. This undermines art work, music and public speaking skills in students. A good curriculum is transparent and it values interactions out of school (Freedman, 2003). In this way, it leads to developing new knowledge and improving existing knowledge. It values diversity of teaching methods and flexibility of teachers. Teachers are able to use objects, drawings, videos, nature walks, park visits and photographs to teach instead of using course books only. Five questions that can be used for discussion of the chapter are; what is the importance of a democratic curriculum? Is it important to teach cultural diversity? What are the pertinent issues surrounding visual culture? Who enhances knowledge construction among students? Should students be involved in designing

Plasmid Retention and Bacteria Growth in E Coli

Plasmid Retention and Bacteria Growth in E Coli The increasing interest by the industry in recombinant protein production has caused an intensive study in this area during the last years. However, it is well known that there are a number of issues associated with the high expression of a recombinant protein. E. coli is one of the most used organisms for this purpose. In this organism, the most common and challenging problem is the formation of inclusion bodies. Probably, an incorrect folding process provokes that the recombinant protein forms those structures. When the protein forms inclusion bodies, it is insoluble and usually useless. In order to find a proper protocol for the high production of the protein S, we have assessed the expression system which use the BL21*DE3 strain as host and the pCV05 plasmid which contains the protein S sequence fused with the His tag sequence. Growth rate, plasmid loss and recombinant expression level were assessed. We obtained a reasonable production of target protein in the insoluble fraction. Further research is needed to know whether the processing of the His tag is able to make soluble the protein from the inclusion bodies as is described by other researchers. Introduction The increasing interest by the industry in recombinant protein production (RPP), due to the number of applications it can provide, has caused an intensive study in this area in order to improve its protocols. Thus, an improvement would make possible an increment in the target protein yield and the quality production as well as to establish more efficient host and plasmid for each target protein . The most common hosts utilized in RPP are bacteria because of the capacity that they have to express almost any gen and the relative facility to modify and use their plasmids in order to produce the target protein . However, it is widely known the number of problems that these hosts have when they produce a high amount of recombinant protein. Firstly, a frequent problem is the appearance of inclusion bodies which hinder a correct recovery of the target protein produced . Secondly, the host lysis event is the other common problem in RPP in bacteria. This undesirable happening in the production of recombinant proteins can be produced for several reasons. One of them is the high level synthesis of the mRNA and the target protein . Other reasons described are the accumulation of fragments of the recombinant protein because of the proteolysis . Finally, the main cause of the problems related to RPP is the accumulation of incorrectly folded intermediates of the recombinant protein. In E. coli this fact implies general stress responses . In order to find a proper protocol for the protein S (PS) production in E. coli and know more about the RPP process, we conducted an experiment testing post-induction bacteria growth, production of target protein and plasmid retention. In this experiment the E. coli strain BL21*DE3 transformed with the plasmid pCV05 was used to express the PS fused with a C-terminal His tag (6xHis). Materials and Methods The E. coli strain BL21*DE3 transformed with the plasmid pCV05 (a derivative of pET21a plasmid) was utilized to conduct the experiment. This plasmid carries in its sequence the gene of PS fused with a C-terminal extra sequence which encodes a His tag (predicted molecular weight 60 KDa). A flask with 25 mL LB (10 g L-1 tryptone, 5 g L-1 yeast extract, 5 g L-1 NaCl) supplemented with 0.1 g L-1 carbenicillin was inoculated with a single colony of E. coli. Subsequently, this flask was incubated during 7 hours (25 °C). The next step was to inoculate the 2.5-L fermenter (ΑG CH-4103 Bottmingen ®), which contained 2 L LB supplemented with 0.5% (w/v) glucose and 0.1 g L-1 carbenicillin, with 25 mL from the flask previously inoculated and incubated during 8 hours (25 °C). Immediately before inoculate the fermenter, a sample was taken in order to use it as a blank to measure the OD650nm of the following samples. The culture was grown at 25 °C until an OD650 nm of nearly 0.6 when it was added the inducer of recombinant protein expression (IPTG). The culture was supplemented with 100  µM IPTG. After this step, the culture was grown at 25 °C during 8 hours. The pH was controlled at 7 adding 5% (v/v) HCl and 1 M NH3, and 0.03% (v/v) final concentration of silicone antifoam was added in the fermenter due to prevent foaming during the last hours of the fermentation. During that period of 8 hours, the culture was fed and samples were taken at various times. One sample was picked just before the induction (0h) and the other samples were collected at 1h, 3h, 4h, 5h, 6h and 7h after the induction in order to check the bacteria growth. Besides, a pellet of bacteria was obtained from those samples collected to check the amount of target protein by a 15% SDS-PAGE gel subsequently stained with 0.2% (w/v) Coomassie Blue using the NEB Prestained Protein Marker, Broad Range (7-175 KDa) ® as a marker. In addition, BugBuster Protein Extraction Reagent ® protocol was cond ucted for additional 0h, 3h, 4h and 8h samples due to separate the insoluble and the soluble protein fractions from the bacteria and assess them by SDS-PAGE as described above. On the other hand, 0h, 3h and 7h samples were plated onto non selective nutrient agar (NA) and later replicated onto NA supplemented with 0.1 g L-1 carbenicillin in order to assess the plasmid retention of the bacteria. Results Inducer effect in Bacteria growth It was conducted a measurement of OD650 nm of the culture during the 8h-period of growth. In order to conduct that assessment, samples were collected immediately before the induction (0h) and 1h, 3h, 4h, 5h, 6h, 7h after the induction with IPTG. After the IPTG induction the culture grew slowly until time 3h. After that time, the growth rate was increased significantly until time 5h. However, at this time the culture presented a decrease in growth until time 7h. The growth dropped specially between time 5h and 6h (Fig. 1). Plasmid retention To assess how the E. coli strain BL21*DE3 retains the pCV05 plasmid after the IPTG induction, samples from the fermenter were collected at time 0h, 3h and 7h post-induction. Serial dilutions of these samples were plated onto non selective NA and subsequently replicated onto NA supplemented with carbenicillin. The relation between the colonies grown on NA plates and the colonies grown in NA supplemented with carbenicillin give the % of plasmid retention. The plasmid retention was hardly altered during the period of 7 hours post-induction assessed. Unexpectedly, according to the results obtained, the plasmid retention 3 hours after the induction is higher than the rate at time 0h. Regarding the time 7h, the plasmid retention percentage is much lower than in time 0h and 3h (Fig. 2). Target protein yield In order to compare the target protein yield during the growth of the culture, samples were collected immediately before the induction (0h) and different times post-induction (1h, 2h, 3h, 4h and 5h). In addition, insoluble and soluble fractions for time 0h, 3h, 4h and 8h were obtained in order to know if the target protein was correctly folded (protein in soluble fraction) or incorrectly folded (insoluble fraction). These samples were assessed in a SDS-PAGE gel. According to the SDS-PAGE profiles, although the PS::His band should be around 60 KDa, in this experiment the target band seems to be below the expected weight (Fig. 3). Regarding the total protein assessment, this band shows a gradual increase in its intensity from time 3h to time 5h post-induction. However, at time 0h, 1h, 2h after induction there was not significant production of the recombinant protein (Fig. 3a). As for the soluble and insoluble fraction samples, the SDS-PAGE analysis revealed a higher intensity of the target protein band in the insoluble fraction than in the soluble fraction of the times 8h, 4h and 3h. At those times, the intensity of the recombinant protein band for insoluble fraction samples is almost impossible to appreciate. The same occurs for the soluble and insoluble fraction samples at time 0h (Fig. 3b). Overall, according to the results of the SDS-PAGE profiles, there was a significant recombinant protein production since the time 3h after the induction with IPTG. On the other hand, it seems to be that there was not a proper target protein folding because the recombinant protein could be found in the insoluble fraction rather than in the soluble fraction. Discussion The main cause of an incorrect folding of the recombinant protein when a high yield is conducted is well known. The accumulation of misfolded protein intermediates causes considerable stress in the host cell . A wide range of different strategies have been conducted in order to solve this problem . It has been described that the use of IPTG-inducible T7 RNA polymerase system in the BL21 strain to produce high concentrations of recombinant protein usually implies a high level of post-induction stress . The solution proposed by some researchers is select mutants which have lower expression rates of the recombinant protein . Other researchers have opted for limiting the concentration of inducer used . Nowadays, the establishment of general protocols and host for the different target proteins is still a challenge. In this experiment, we have assessed the capacity of the E. coli strain BL21*DE3 to produce a high amount of PS fused with a C-terminal His tag as well as the bacteria growth during the process and the plasmid retention. According to the first part of the results, the bacteria growth rate changed during the 7 hours of fermentation. It is described that the high amount of recombinant protein in the cell causes stress response. This stress response implies that the growth rate of the culture turned into a negative rate (the number of cells in the culture decreases) . The data collected from other groups conducting the same experiment shows that this event has happened in 2 groups but the other 3 groups have a different growth pattern (Fig. 4). Technical issues may explain this incongruity between the different results obtained. Regarding the plasmid retention, the data obtained in this experiment suggest that because of the stress suffered by the cells when the recombinant protein levels are higher, the cells tend to have a lower rate of plasmid retention. An explanation may be that the bacteria with the plasmid suffer a higher stress due to the induction by the IPTG, and thus, they have less chance to survive than the bacteria which accidentally do not have the plasmid. Therefore, the bacteria suffer a selective pressure which results in a plasmid loss and it is more obvious after several hours of growth. Analyzing the data obtained by other colleagues, it is supported that there is a relation between the time after the induction and the plasmid retention rates (Table 1). As for the PS::His yield, the data shows that in the BL21*DE3 strain using pCV05 as a plasmid and with the conditions described before, this expression system needs 3 hours to start expressing the recombinant protein. After that time, it seems that the most part of the target protein is in the insoluble fraction. Regarding the total protein samples assessment, the relative amount of target protein produced by the cultures of the all groups was the same (Table 2). However, the recombinant protein is still in the insoluble fraction after the BugBuster Protein Extraction Reagent protocol. Probably, some of the proteins from the insoluble fraction were forming inclusion bodies . It is described that after the expression of the protein removing the His tag makes the recombinant protein more soluble and thus, it is possible to dissolve the inclusion bodies and recover a functional recombinant protein . Furthermore, the His tag allows an easier purification of the protein due to the affinit y of this polypeptide for metal ions . In order to confirm those statements for PS, it would be necessary to conduct purification and a proteolysis process of the His tag in order to assess whether the efficiency of this expression system is cost-effective and the amount of protein obtained is enough. The conclusion of these data is that this expression system for the PS yield could be a good and profitable system whether the His tag added finally allows to recover the recombinant protein from the inclusion bodies. Acknowledgements We are grateful to Dr Claire Vine, Dr Ian Cadby and Dr Jeff Cole for the excellent support given as well as the rest of the groups which conducted the same experiment because they have contributed to the experiment with very valuable data.

Biography of Charles Cromwell :: English Civil War Generals Military Essays

Biography of Charles Cromwell As a general on the parliamentary side of the English Civil War vs. Charles I, Cromwell helped bring about the overthrow of the Stuart monarchy, and he raised his country's status to that of a leading European power since the death of Queen Elizabeth I. Being a man with strong character made him one of the most remarkable rulers in modern European history. Although he was a convinced Calvinist he believed deeply in the value of religious toleration. Cromwell's victories at home and abroad helped to vitalize a Puritan attitude of mind, in Great Britain and in North America, which has continued to influence political and social life until recent times. (Gaunt, 1996) Cromwell, the only son of Robert Cromwell and Elizabeth Steward was born in Huntingdon, England in 1599. His father, who was active in local affairs, had been a member of one of Queen Elizabeth's parliaments. Robert Cromwell died when his son was 18, but his widow lived to the age of 89. Oliver went to the local grammar school and then for a year attended Sidney Sussex College, Cambridge. After his father died he left Cambridge to go care for his mother and sisters but it is believed that he studies at Lincoln's Inn in London, where gentlemen could acquire a smattering of law. In 1620 he married Elizabeth, daughter of Sir James Bourchier, a merchant in London. They had five sons and four daughters. (Kathe, 1984) Both his father and mother were Protestants who had profited from the destruction of the monasteries during the reign of King Henry VIII, and they probably influenced their son in his religious upbringing. Both his schoolmaster in Huntingdon and the Master of Sidney Sussex College were enthusiastic Calvinists and strongly anti-Catholic. In his youth Cromwell was not very studious, since he enjoyed outdoor sports, such as hunting; but he was an avid reader of the Bible, and he admired Sir Walter Raleigh's The History of the World. Cromwell learned that the sins of man could be punished on earth but that God, through His Holy Spirit, could guide the elect into the paths of righteousness. (Kathe, 1984) In the early parts of his married life Cromwell, like his father, was quite conscious of his responsibilities to his fellow men and concerned himself with affairs in his native fenlands, but at the same time he had a spiritual and psychological struggle which confused him and damaged his health.

Ellis Island Immigration Essay

Family came, Haiti origin, along with 15 year old daughter for crises counseling and to file a petition. The daughter lived in United States since she was 5 years old. She also had siblings who are way older then she is, who were brought up back in Haiti. Parents complain that she is disobedient and they wanted to put her in jail. What they called disobedient is her refusing to take earrings off, because in their culture females are not allowed to use anything that might make them look attractive (at least that’s what they say). Parents are culture orientated and very traditional people. I talked to girl alone for long time, she felt misunderstood on so many levels. Nothing was wrong with her psychologically, and there was no need for psychiatric evaluation at all. She seemed very smart young female, who is also a typical teenager and her parents had hard time dealing with that. She want it make up, go to movies, and her parents didn’t like it. She skipped school from time to time, but nothing serious so there was no need to file petition for truancy. Both me, and psychologist I worked with had counseling with her parents. They kept telling us â€Å"other children were never like this,† so I tried to explain them that other children were raised in a different environment and off course they are going to act differently. I also told them I cannot file a petition because 15 year old has earrings on. It was a tough one, and we knew it would take time to change perspective of her parents. This is why Ellis Island immigration is so significant to me. Being labeled â€Å"insane† for something that is totally acceptable in own culture is pure injustice. Ellis Island is the place where everything started, and this occurs when people begun to be seen as the same but in fact, different. We all may have been equal, but have different values and beliefs and this has to be seen by everybody, especially professionals in such field. References Cohen, R. , & Swerdlik, M. (2005). Psychological Testing and Assessment: An Introduction to Tests and Measurement (6th ed. ). , : The McGraw-Hill Companies. Ellis Island http://sydaby. eget. net/swe/ellis_island. htm Ellis Island www. history. com

Spongebob Square Pants has Many Attributes of the Greek...

â€Å"Hes not a child but hes childlike, hes not a grown up, hes not a kid, maybe he sounds like an elf on helium, well play with it† (Tom Kenney). Although, the television show SpongeBob Squarepants is known for not taking anything seriously, the characters are made of substance and real human attributes. Characteristics like greed, power, and deception are all recognizable in Greek gods from ancient mythology as well as in Mr. Krabs, King Neptune, and Plankton. Greek gods and the characters of SpongeBob, as unlikely as this may seem, are similar because each person is represented by one core characteristic. The gods Triton, Hades, Koalemos, Poseidon, and Midas would make the perfect dream team for the television show SpongeBob†¦show more content†¦The more blunt side to Patrick relates to â€Å"The god of silliness and stupidity† (Greek Mythology). If Patrick Star has an I.Q. it is a negative number. Also, Patrick is the reason for all of the problems S pongeBob has to solve for him. Patrick creates mayhem for his friends just like Koalemos creates mayhem for his fellow gods (Theoi Koalemos). The sensitive and caring side of Patrick corresponds with Philotes, â€Å"the personification of affection and friendship† (Greek Myth Index). Out of all the factors making up the characterization of Patrick Star, the friendship he has with SpongeBob stands out the most. When talking about what Patrick does when SpongeBob is at work Patrick says, â€Å"I wait for you to come back† (spongybob). The only thing Patrick can truly focus on is friendship and loyalty. The personality of Patrick Star works well with Koalemos and Philotes. Eugene Krabs, owner of the Krusty Krab, relates well to the Greek king Midas. King Midas is known for his immense greed and wisdom (Greek Myths). King Midas had found favor in the gods so â€Å"God Dionysus was pleased with Midas and his decision not to punish Silenus, thus he asked Midas what he wanted the most from his life- in order to return the favor† (Greek Myths). Midas’ greed overwhelms him when he asks for everything he touches to turn to gold (Greek Myths). Mr. Krabs is characterized by this kind of extreme greed because of the way he overprices everything he