Essay on Abraham Lincoln the Politician - 1473 Words

Abraham Lincoln the Politician Abraham Lincoln: Moral, Just, and Practical Today in the United States one of the most controversial issues is race relations. Mainstream America would like to exist in an environment of integration and race independent societal construct. However, one cannot negate the existence of racially motivated problems. Slavery, which is a primary component of the development of what we call modern American culture and its abolition could be considered the moment where race relations started to become an issue in the US. Slavery was abolished after the civil war by President Abraham Lincoln. The question becomes, was the blood shed in the civil war motivated by a moral imperative to end†¦show more content†¦He built his own flatboat, navigated it to New Orleans, and then settled for a time to manage a store and a mill, and act as Post Master, as well as studying Law. In 1836 he was licensed to practice as an attorney, and settled in the following year in Springfield, Illinois, where he married Mary Todd. Though clashes of temperament between the high tempered Mary and her sincere but often inconsiderate husband meant that the marriage was never an easy one, it was not the tragedy that legend has made it. In 1847 Lincoln was elected to congress as a Whig, but he did not particularly distinguish himself in his one term of office, which was chiefly valuable for the formation of political associations and the establishment of a reputation for sincerity and honesty. He was not reelected, and it was an ebb-period of his career. It was not until 1854 that he began to stand out from the hundreds of provincial lawyer-turned-politician. In the last year the Bilk to reopen the Louisiana purchase to slavery gave rise to much northern opposition, and to the birth of the new Republican party. Lincolns speech at Peoria was the first occasion on which he stated clearly his personal attitude to the slavery issue. Slavery is founded in the selfishness of mans nature -- opposition to it is his love of justice. In 1856 he joined the Republican party, and fairly launched himself on his new career in 1858 when he stoodShow MoreRelatedAbraham Lincoln: Great Emancipator or Common Politician?1487 Words   |  6 PagesPresident Abraham Lincoln has been revered as one of the greatest presidents in the history of the United States. He is known for his great effect on slavery and served his terms during the civil war in a time of great controversy. The American Civil War (1860-1865) occurred at the exact time of Lincolns presidency (1861-1865). The North and the South were divided and a big issue was slavery, on which Lincoln took an anti-slavery stance. Lincoln has been called many things because of his views fromRead MoreSlavery During The 19th Century895 Words   |  4 PagesStates was split into two sections, the north and south, over the dispute of slavery. The south sought to further slavery while the north was in favor of abolishing slavery. Around 1858, Abraham Lincoln and Stephen Dougla s, two politicians with completely different views on slavery fought each other with politicians trying to win the presidency election of 1860. This was known as the â€Å"Great Debates of 1858†. Slavery during the late 1800’s was at its highest peak; almost 1 out of 4 citizens had slavesRead MoreMaking An Abraham Lincoln Movie901 Words   |  4 Pages Making an Abraham Lincoln movie can be very tricky and a difficult job. Everybody knows the story of Abraham Lincoln and knows the persona that President Lincoln can give out. Spielberg’s, Ford’s, and Griffith’s Lincoln all did very notable work. Griffith’s Lincoln in Birth of a Nation was a worthy performance by Henabery. Ford’s Young Mr. Lincoln was known as one great over all film. The scenes in the film were spot on and the Lincoln played by young Mr. Henry Fonda was the best in the businessRead MoreAbraham Lincoln And The Gettysburg Address1152 Words   |  5 PagesAbraham Lincoln was born on Feb. 12, 1809, in Hardin, now known as larue, County Kentucky. Lincoln got married in 1842 to Mary Todd. His children were Robert Todd, Edward Baker, William Wallace, and Thomas III. A braham was in office from 1834-1842. Abraham Lincoln died at age 56, april 15, 1865. The Republican Party met in Chicago to select a candidate. They took the chance now because of the democrats in turmoil. They needed someone who would help the north and also win most of the electoral collegesRead More Abraham Lincoln Essay1464 Words   |  6 Pages Abraham Lincoln sprang to the presidency from extremely humble and tragic roots. His father, Thomas, and his mother, Nancy, were both illiterate. When Abraham was young, he had a sister who died as an infant and a brother who died as a boy. When Abraham was nine years old, his mother tragically died, leaving him in the care of only his father, who, within the year of his wife’s death, remarried a widow, Sarah Bush Johnston. Sarah was extremely kind to Lincoln and encouraged his developing love ofRead MoreAbraham Lincoln And The Civil War1095 Words   |  5 Pages1861 Abraham Lincoln became the sixteen president of the United States. He had the Proclamation of declaring forever to free slaves within the Confederac y in the year 1863. Lincoln directly told the South In your hands, my dissatisfied fellow countrymen, and not in mine, is the momentous issue of civil war. The government will not assail you.... You have no oath registered in Heaven to destroy the government, while I shall have the most solemn one to preserve, protect and defend it. Lincoln wasRead MoreLincoln and Good Politics Essay946 Words   |  4 PagesLincoln and Good Politics Politicians today as well as yesterday must appeal to the masses to have any chance at being elected into office. Leading up to and during the election of 1860, with tensions rising between the North and South, the issue of slavery was a key to winning the election. Abraham Lincoln had repeatedly affirmed that Congress had no constitutional right to interfere with slavery in the South (Enduring Vision, p. 399). This modest view is what won him the RepublicanRead MoreEssay Abraham Lincoln1426 Words   |  6 Pages As the 16th president, Abraham Lincoln was known to be the greatest American President. He guided his country through one of the most catastrophic experiences in its history, the Civil War. He was a very unique president, being the first president to have a beard, being the tallest president standing at 6’4 and also being the first to be assassinated. Abe Lincoln accomplished many things in the 4 year term he had served before being assassinated. With his intelligence and hard work, he madeRead MoreThe Life Of Abraham Lincoln754 Words   |  4 PagesValenzuela 1 Issac Valenzuela Mrs. Barr 7th Grade Literature May 24, 2016 The Life of Abraham Lincoln Abraham Lincoln was born and raised in a log cabin. He was born on February 12, 1809. His mother was a great Christian. She often told them stories from the Bible. One day, Lincoln’s mother died of poisoned milk when he was only nine. Abraham Lincoln was one of the tallest presidents in our history. He was six feet, four inches tall. When he was elected president, he started theRead MoreAbraham Lincoln, Slavery and the American Civil War Essay1716 Words   |  7 Pagesinvestigation will analyze how Abraham Lincolns view on slavery reflected during and after the American Civil War from 1861 to 1865. To analyze exactly how Lincolns position on slavery affected the war overall, this investigation looks at Lincolns moral and religious views as well as his social and political views. Two main sources were used, both dealing with events relevant to his political career and his roots in his career and other important issues including slavery. Lincoln by David Herbert Do nald

David Beckham free essay sample

This is an essay will be about David Beckham the English soccer player. David is one of the best players in the world. There are several aspects about Beckham`s life. His soccer career, he became more than a usual football player and his personal life. That was the introduction. Now let’s move to the main part. David played in many clubs around the world. However, his most crucial stage of development and reputation happened when he was playing for two of the best Five soccer clubs in the world which are Manchester United from 1993 to 2003 and Real Madrid from 2003 to 2007. Therefore, he won many of championships and individual awards. For instance, he won the Union of European Football Associations (UEFA) Champions league with Manchester United, La Liga with Real Madrid and England player of the year in 2003. Also, he played as a loan player in Ac Milan that is one of the top clubs around the world too. We will write a custom essay sample on David Beckham or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page In addition, in 2007 Beckham had the highest transfer price in his career life when he transferred from Real Madrid to Los Angeles Galaxy by $ 32. 5 million in five years. Beckham became more than an average player. He did not stop just as a famous player, he was smart to use his fame well using. For example, he opened a football academy in London and Los Angeles. Likewise, he became one of the most wanted famous people in the advertising world. He did a large number of advertising for a lot of famous companies such as Pepsi and adidas. Furthermore, Beckham became a model in many famous clothing companies around the world. In 1997, Beckham start dating with the famous singer Victoria, who was one of the spice girls music group. The result for this successful relationship they get married in 1999, at Luttrellstown Castle in Ireland. Now David and Victoria have four children(three sons and a daughter). In conclusion, this essay was about David Beckham the English footballer. It has showed, his football career, Beckham did not stop only as a normal soccer player and his personal life.

Friends Can Bring You Down free essay sample

â€Å"I believe that friends can bring you down, just as much as you hoped they wouldn’t.† I’m almost a 15 year old teenage girl just wanting to be able to make old things better and throw all the feelings away like they don’t matter. It’s been a while now since me and my three other BFF’S (best friends forever) have talked, walking by myself around in the halls and seeing them still together, laughing and not caring that I’m falling apart, tears me down even more than just a little argument that was over in less than five minutes. While sitting at lunch or just floating around all the rumors going on and having the automatic thought â€Å"It’s them†. I don’t understand why if they told me they would always be there when I’m falling apart or need a shoulder to cry on, when they haven’t ever before. We will write a custom essay sample on Friends Can Bring You Down or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page â€Å"Wouldn’t that make you wonder?† knowing the fact that I’m alone and having to move on is also hard to keep my focus. I remember staying the night having slumber party’s was the only thing we all wanted to do every weekend, all niters laughing as hard as we could at nothing. But all I have now is just memories, memories that I think they all forgot about, just like they forgot about me or that I’m still even here. All I have left to do is move on, and not feel sorry anymore there are two other girls Emily (Bonita) and Brittany (Bonita Blonde) that I still have and can have fun with!!! Instead of letting friends bring me down. There’s still a future I believe that I have and I don’t need to sit in my room all the time just to let them feel they have all the power over me. So moving on and finding a way to get over the fact of them not around is gonna take some guts, but I can do it. I think the first thing I’ll try to do is apologize for whatever I did to get it off my chest and let them know how I’m feeling. Cause if you’re like me and you thing you’ve got a good friendship think again or look out because â€Å"friends can bring you down just as much as you hoped they wouldn’t and that’s what I truly believe.†

Scapegoat, Scapegoating, and Scapegoat Theory

Scapegoat, Scapegoating, and Scapegoat Theory Scapegoating refers to a process by which a person or group is unfairly blamed for something that they didnt do and, as a result, the real source of the problem is either never seen or purposefully ignored. Sociologists have documented that scapegoating often occurs between groups when a society is plagued by long-term economic problems or when resources are scarce. Scapegoat theory is used in sociology and psychology as one way to intercept conflict and prejudice between individuals and groups. Origins of the Term The term scapegoat has Biblical origins, coming from the Book of Leviticus. In the book, a goat was sent into the desert carrying the sins of the community. So, a scapegoat was originally understood as a person or animal that symbolically absorbed the sins of others and carried them away from those who committed them. Scapegoats and Scapegoating in Sociology Sociologists recognize four different ways in which scapegoating takes place and scapegoats are created. Scapegoating can be a one-on-one phenomenon, in which one person blames another for something he/she or someone else did. This form of scapegoating is common among children, who blame a sibling or a friend for something they did, to avoid the shame of disappointing their parents and the punishment that might follow a misdeed.Scapegoating also occurs in a one-on-group manner, when one person blames a group for a problem they did not cause: wars, deaths, financial losses of one kind or another, and other personal struggles. This form of scapegoating may sometimes be unfairly blamed on racial, ethnic, religious, class, or anti-immigrant biases.Sometimes scapegoating takes a group-on-one form, when a group of people singles out and blames one person for a problem. For example, when the members of a sports team blame a player who made a mistake for the loss of a match, though other aspects of play also affected the outcome. Or, when someone who alleges an assault is then scapegoated by me mbers of the community for causing trouble or ruining the life of the attacker. Finally, and of most interest to sociologists, is the form of scapegoating that is group-on-group. This occurs when one group blames another for problems that the groups collectively experience, which might be economic or political in nature- like blaming a particular party for the Great Depression (1929-1939) or the Great Recession (2007-2009). This form of scapegoating often manifests across lines of race, ethnicity, religion, or national origin. The Scapegoat Theory of Intergroup Conflict Scapegoating of one group by another has been used throughout history, and still today, as a way to incorrectly explain why certain social, economic, or political problems exist and harm the group doing the scapegoating. Some sociologists say that their research shows that groups that scapegoat occupy a low socio-economic status in society and have little access to wealth and power. They say these people are often experiencing prolonged economic insecurity or poverty, and come to adopt shared outlooks and beliefs that have been documented to lead to prejudice and violence. Sociologists who embrace socialism as a political and economic theory argue that those in a low socioeconomic status are naturally inclined to scapegoat due to the unequal distribution of resources within the society. These sociologists place blame on capitalism as an economic model and exploitation of workers by a wealthy minority. However, these are not the viewpoints of all sociologists. As with any science involving theories, study, research, and conclusions- its not an exact science, and therefore there will be a variety of viewpoints.

Social Phenomena Essay Example | Topics and Well Written Essays - 3000 words

Social Phenomena - Essay Example Each of these views relies upon more or less legitimate arguments and evidence, but neither of them is flawless. Functionalist perspective still remains arguably the most influential tool for understanding the phenomenon of deviant behavior. Since the term 'deviant behavior' is rather broad, only two types of social deviance will be closely analyzed within this project: crime and suicide. Functionalist explanation of the causes and social outcomes of these types can lead us to understanding of functionalist perspective on deviant behavior in general. Emile Durkheim, the famous French sociologist of the 19th - 20th century, was probably the first scholar to adopt the logic of the functional approach studying the phenomenon of deviant behavior. Thus, Durkheim claimed, "When . . . the explanation of a social phenomenon is undertaken, we must seek separately the efficient cause which produces it and the function it fulfills. We must determine whether there is a correspondence between the fact under consideration and the general needs of the social organism, and in what this correspondence consists" (Durkheim, 1930 in Coser, 1977: 143). Ut Utilizing these principles Durkheim focused on the way a society stimulates individuals demonstrate deviant behavior than on individual characteristics of those whom demonstrate such behavior, though majority of sociologists of those days viewed crime, suicide, and other forms of social deviance as an outcome of inborn psychological characteristics of the individual. Durkheim considered such approach flawed. Instead, the scholar argued that deviance in general and crime in particular was by no means abnormal or dangerous for the society: "Crime is normal, an inevitable and necessary part of every society [although] it may take abnormal forms, such as when the crime rate is unusually high (Durkheim, 1961: 872). Durkheim's views on another form of deviance - suicide - were absolutely the same. "La Suicide" was the third major work of Emile Durkheim. This book was the first sociological research that established the traceable empiric connection between the phenomenon of individual suicide and social environment, and defined certain social functions of suicide. In the end of the 19th century suicide or self-destruction was considered a form of deviant behaviour that occurred in insane individuals. Durkheim challenged this traditional standpoint that suicide was an unpreventable pathology. Instead, he provided " a sociological explanation for a phenomenon traditionally regarded as exclusively psychological and individualistic" (Thompson, 1982: 109). In fact, Durkheim formulated the founding principles of functionalist perspective on deviance precisely in his study of suicide. Even today, despite abundance of recent studies, Durkheim's work remains the most significant sociological analysis of s uicide in modern societies. Suicide is a complex phenomenon that can be caused by numerous factors. Many reasons both individual and social have been demonstrated to have certain correlation with self-destructive

Curriculum as process Assignment Example | Topics and Well Written Essays - 250 words

Curriculum as process - Assignment Example The main goal of this transformation was to help students understand democracy and all that it encompasses (Freedman, 2003). Furthermore, it would enable them to appreciate the unity in cultural diversity. Several methods can be used to develop and sustain a democratic curriculum. These include; teaching about conflicts and discussion of issues in open forums by students whereby interests of all individuals should be considered. It is also good to conceptualize curriculum as one process that is concerned with learning in classrooms and outside classroom. Sometimes, schools concentrate on learning in classroom ignoring co-curricular activities. This undermines art work, music and public speaking skills in students. A good curriculum is transparent and it values interactions out of school (Freedman, 2003). In this way, it leads to developing new knowledge and improving existing knowledge. It values diversity of teaching methods and flexibility of teachers. Teachers are able to use objects, drawings, videos, nature walks, park visits and photographs to teach instead of using course books only. Five questions that can be used for discussion of the chapter are; what is the importance of a democratic curriculum? Is it important to teach cultural diversity? What are the pertinent issues surrounding visual culture? Who enhances knowledge construction among students? Should students be involved in designing

Plasmid Retention and Bacteria Growth in E Coli

Plasmid Retention and Bacteria Growth in E Coli The increasing interest by the industry in recombinant protein production has caused an intensive study in this area during the last years. However, it is well known that there are a number of issues associated with the high expression of a recombinant protein. E. coli is one of the most used organisms for this purpose. In this organism, the most common and challenging problem is the formation of inclusion bodies. Probably, an incorrect folding process provokes that the recombinant protein forms those structures. When the protein forms inclusion bodies, it is insoluble and usually useless. In order to find a proper protocol for the high production of the protein S, we have assessed the expression system which use the BL21*DE3 strain as host and the pCV05 plasmid which contains the protein S sequence fused with the His tag sequence. Growth rate, plasmid loss and recombinant expression level were assessed. We obtained a reasonable production of target protein in the insoluble fraction. Further research is needed to know whether the processing of the His tag is able to make soluble the protein from the inclusion bodies as is described by other researchers. Introduction The increasing interest by the industry in recombinant protein production (RPP), due to the number of applications it can provide, has caused an intensive study in this area in order to improve its protocols. Thus, an improvement would make possible an increment in the target protein yield and the quality production as well as to establish more efficient host and plasmid for each target protein . The most common hosts utilized in RPP are bacteria because of the capacity that they have to express almost any gen and the relative facility to modify and use their plasmids in order to produce the target protein . However, it is widely known the number of problems that these hosts have when they produce a high amount of recombinant protein. Firstly, a frequent problem is the appearance of inclusion bodies which hinder a correct recovery of the target protein produced . Secondly, the host lysis event is the other common problem in RPP in bacteria. This undesirable happening in the production of recombinant proteins can be produced for several reasons. One of them is the high level synthesis of the mRNA and the target protein . Other reasons described are the accumulation of fragments of the recombinant protein because of the proteolysis . Finally, the main cause of the problems related to RPP is the accumulation of incorrectly folded intermediates of the recombinant protein. In E. coli this fact implies general stress responses . In order to find a proper protocol for the protein S (PS) production in E. coli and know more about the RPP process, we conducted an experiment testing post-induction bacteria growth, production of target protein and plasmid retention. In this experiment the E. coli strain BL21*DE3 transformed with the plasmid pCV05 was used to express the PS fused with a C-terminal His tag (6xHis). Materials and Methods The E. coli strain BL21*DE3 transformed with the plasmid pCV05 (a derivative of pET21a plasmid) was utilized to conduct the experiment. This plasmid carries in its sequence the gene of PS fused with a C-terminal extra sequence which encodes a His tag (predicted molecular weight 60 KDa). A flask with 25 mL LB (10 g L-1 tryptone, 5 g L-1 yeast extract, 5 g L-1 NaCl) supplemented with 0.1 g L-1 carbenicillin was inoculated with a single colony of E. coli. Subsequently, this flask was incubated during 7 hours (25 °C). The next step was to inoculate the 2.5-L fermenter (ΑG CH-4103 Bottmingen ®), which contained 2 L LB supplemented with 0.5% (w/v) glucose and 0.1 g L-1 carbenicillin, with 25 mL from the flask previously inoculated and incubated during 8 hours (25 °C). Immediately before inoculate the fermenter, a sample was taken in order to use it as a blank to measure the OD650nm of the following samples. The culture was grown at 25 °C until an OD650 nm of nearly 0.6 when it was added the inducer of recombinant protein expression (IPTG). The culture was supplemented with 100  µM IPTG. After this step, the culture was grown at 25 °C during 8 hours. The pH was controlled at 7 adding 5% (v/v) HCl and 1 M NH3, and 0.03% (v/v) final concentration of silicone antifoam was added in the fermenter due to prevent foaming during the last hours of the fermentation. During that period of 8 hours, the culture was fed and samples were taken at various times. One sample was picked just before the induction (0h) and the other samples were collected at 1h, 3h, 4h, 5h, 6h and 7h after the induction in order to check the bacteria growth. Besides, a pellet of bacteria was obtained from those samples collected to check the amount of target protein by a 15% SDS-PAGE gel subsequently stained with 0.2% (w/v) Coomassie Blue using the NEB Prestained Protein Marker, Broad Range (7-175 KDa) ® as a marker. In addition, BugBuster Protein Extraction Reagent ® protocol was cond ucted for additional 0h, 3h, 4h and 8h samples due to separate the insoluble and the soluble protein fractions from the bacteria and assess them by SDS-PAGE as described above. On the other hand, 0h, 3h and 7h samples were plated onto non selective nutrient agar (NA) and later replicated onto NA supplemented with 0.1 g L-1 carbenicillin in order to assess the plasmid retention of the bacteria. Results Inducer effect in Bacteria growth It was conducted a measurement of OD650 nm of the culture during the 8h-period of growth. In order to conduct that assessment, samples were collected immediately before the induction (0h) and 1h, 3h, 4h, 5h, 6h, 7h after the induction with IPTG. After the IPTG induction the culture grew slowly until time 3h. After that time, the growth rate was increased significantly until time 5h. However, at this time the culture presented a decrease in growth until time 7h. The growth dropped specially between time 5h and 6h (Fig. 1). Plasmid retention To assess how the E. coli strain BL21*DE3 retains the pCV05 plasmid after the IPTG induction, samples from the fermenter were collected at time 0h, 3h and 7h post-induction. Serial dilutions of these samples were plated onto non selective NA and subsequently replicated onto NA supplemented with carbenicillin. The relation between the colonies grown on NA plates and the colonies grown in NA supplemented with carbenicillin give the % of plasmid retention. The plasmid retention was hardly altered during the period of 7 hours post-induction assessed. Unexpectedly, according to the results obtained, the plasmid retention 3 hours after the induction is higher than the rate at time 0h. Regarding the time 7h, the plasmid retention percentage is much lower than in time 0h and 3h (Fig. 2). Target protein yield In order to compare the target protein yield during the growth of the culture, samples were collected immediately before the induction (0h) and different times post-induction (1h, 2h, 3h, 4h and 5h). In addition, insoluble and soluble fractions for time 0h, 3h, 4h and 8h were obtained in order to know if the target protein was correctly folded (protein in soluble fraction) or incorrectly folded (insoluble fraction). These samples were assessed in a SDS-PAGE gel. According to the SDS-PAGE profiles, although the PS::His band should be around 60 KDa, in this experiment the target band seems to be below the expected weight (Fig. 3). Regarding the total protein assessment, this band shows a gradual increase in its intensity from time 3h to time 5h post-induction. However, at time 0h, 1h, 2h after induction there was not significant production of the recombinant protein (Fig. 3a). As for the soluble and insoluble fraction samples, the SDS-PAGE analysis revealed a higher intensity of the target protein band in the insoluble fraction than in the soluble fraction of the times 8h, 4h and 3h. At those times, the intensity of the recombinant protein band for insoluble fraction samples is almost impossible to appreciate. The same occurs for the soluble and insoluble fraction samples at time 0h (Fig. 3b). Overall, according to the results of the SDS-PAGE profiles, there was a significant recombinant protein production since the time 3h after the induction with IPTG. On the other hand, it seems to be that there was not a proper target protein folding because the recombinant protein could be found in the insoluble fraction rather than in the soluble fraction. Discussion The main cause of an incorrect folding of the recombinant protein when a high yield is conducted is well known. The accumulation of misfolded protein intermediates causes considerable stress in the host cell . A wide range of different strategies have been conducted in order to solve this problem . It has been described that the use of IPTG-inducible T7 RNA polymerase system in the BL21 strain to produce high concentrations of recombinant protein usually implies a high level of post-induction stress . The solution proposed by some researchers is select mutants which have lower expression rates of the recombinant protein . Other researchers have opted for limiting the concentration of inducer used . Nowadays, the establishment of general protocols and host for the different target proteins is still a challenge. In this experiment, we have assessed the capacity of the E. coli strain BL21*DE3 to produce a high amount of PS fused with a C-terminal His tag as well as the bacteria growth during the process and the plasmid retention. According to the first part of the results, the bacteria growth rate changed during the 7 hours of fermentation. It is described that the high amount of recombinant protein in the cell causes stress response. This stress response implies that the growth rate of the culture turned into a negative rate (the number of cells in the culture decreases) . The data collected from other groups conducting the same experiment shows that this event has happened in 2 groups but the other 3 groups have a different growth pattern (Fig. 4). Technical issues may explain this incongruity between the different results obtained. Regarding the plasmid retention, the data obtained in this experiment suggest that because of the stress suffered by the cells when the recombinant protein levels are higher, the cells tend to have a lower rate of plasmid retention. An explanation may be that the bacteria with the plasmid suffer a higher stress due to the induction by the IPTG, and thus, they have less chance to survive than the bacteria which accidentally do not have the plasmid. Therefore, the bacteria suffer a selective pressure which results in a plasmid loss and it is more obvious after several hours of growth. Analyzing the data obtained by other colleagues, it is supported that there is a relation between the time after the induction and the plasmid retention rates (Table 1). As for the PS::His yield, the data shows that in the BL21*DE3 strain using pCV05 as a plasmid and with the conditions described before, this expression system needs 3 hours to start expressing the recombinant protein. After that time, it seems that the most part of the target protein is in the insoluble fraction. Regarding the total protein samples assessment, the relative amount of target protein produced by the cultures of the all groups was the same (Table 2). However, the recombinant protein is still in the insoluble fraction after the BugBuster Protein Extraction Reagent protocol. Probably, some of the proteins from the insoluble fraction were forming inclusion bodies . It is described that after the expression of the protein removing the His tag makes the recombinant protein more soluble and thus, it is possible to dissolve the inclusion bodies and recover a functional recombinant protein . Furthermore, the His tag allows an easier purification of the protein due to the affinit y of this polypeptide for metal ions . In order to confirm those statements for PS, it would be necessary to conduct purification and a proteolysis process of the His tag in order to assess whether the efficiency of this expression system is cost-effective and the amount of protein obtained is enough. The conclusion of these data is that this expression system for the PS yield could be a good and profitable system whether the His tag added finally allows to recover the recombinant protein from the inclusion bodies. Acknowledgements We are grateful to Dr Claire Vine, Dr Ian Cadby and Dr Jeff Cole for the excellent support given as well as the rest of the groups which conducted the same experiment because they have contributed to the experiment with very valuable data.